I. Objective
Recently, pasted-type pozzolan-based root canal sealer (PPS) (Maruchi, Wonju, Korea) was experimentally developed for use in the root canal sealing process. The aim of this study was to investigate the solubility, radiopacity and biological effects of PPS cement in comparison with mineral trioxide aggregate (MTA) (ProRoot, Dentsply, Tulsa, OK, USA) and resin-based root canal sealer (AHPlus [AHP]; Dentsply DeTrey, Konstanz, Germany).
II. Materials & Methods
The solubility, pH values, dimensional change, and radiopacity of the three tested materials were measured. Biocompatibility was evaluated on the basis of cell morphology and a viability test using osteoblast-like MC3T3-E1 cells. The expression of osteoblast-related genes was evaluated by reverse transcriptionpolymerase chain reaction (RT-PCR).
III. Results
The solubility, pH value, and dimensional change of PPS was similar with those of ProRoot. Scanning electron microscopy revealed that well-spread and flattened cells were observed in close contact with the surfaces of MTA and PPS. In contrast, a few rounded cells were observed on the AHP surface, and no living cells were observed. PR, PPS and AHP showed similar cell viability for 7 days. However, AHP showed lower cell viability after 14 days compared with the other groups. Further, the expression of osteogenicrelated markers was higher in the MTA- and PPS-treated cells when compared with AHP-treated cells.
IV. Conclusion
Our study showed that the PPS exhibited more favorable biocompatibility and osteogenicity compared to a widely used resin-based sealer, AHP. Furthermore, it showed the physical and biological properties comparable with MTA, which is believed as a biocompatible endodontic material. Within the limitation of this study, the PPS has the potential to be used as acceptable root canal sealer in terms of physical and biological properties.
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